Selenite broth tubes, or fecal matter from any stool specimens can be inoculated directly into the broth. Uses advised against no information available details of the supplier of the safety data sheet. Bd diagnostic systems regulatory regulatory documents. Copan selenite broth can be used for processing with automated systems for planting and streaking on culture media plates copan wasp included.
Not all products are available for sale in all territories. You can also split and merge files back together using out free online merge tool. Mwe selenite broth is an enrichment medium for the isolation of salmonella species from faecal specimens. Selenite cystine broth intended use selenite cystine broth is used as a selective enrichment medium for the isolation of salmonella from feces, foods, water and other materials of sanitary importance. It is intended to be used in the clinical microbiology laboratory to process specimens collected using fecal transwabs, or directly using faecal specimens.
Refer to the front of remel technical manual of microbiological media for. Split pdf file separate one page or a whole set for easy conversion into independent pdf files. Strontium chloride and strontium selenite enrichment broth. Selenite cystine broth for microbiology sigmaaldrich. Sodium selenite inhibits grampositive bacteria and most enteric gram. Remel selenite cystine broth is selective enrichment broth for isolation of salmonella spp. Selenite broth is used as a selective medium for the isolation of salmonella species. Selenite broth selenitef broth laboratoires humeau. This medium is prepared according to the original formula described as selenite f broth by leifson and recommended by the american public health association for the examination of food.
Selenite cystine enrichment broth msds material safety data sheet or sds, coa and coq, dossiers, brochures and other available documents. Selenite broth is used as an enrichment medium for the isolation of salmonella and shigella from stool, urine, water and food products. Cled agar is used for the differentiation and enumeration of microorganisms in urine. Wellcolex colour salmonella test and selenitef broth. Inoculate the food sample into recommended preenrichment broth, and then transfer 1 ml of mixture to 10 ml of fluid selenite cystine medium and also to 10 ml tetrathionate broth m032. Selenite cystine broth inhibits the early multiplication of bacteria such as coliforms but allows the salmonellae to grow with ease. Safety data sheet revision date 16feb2015 revision number 1 1. For isolation and enrichment of salmonella from faeces, urine or other pathological. Selenite broth was developed by leifson for enrichment of salmonella from. Selenite broth selenitef broth is used as an enrichment medium for the isolation of salmonella from feces, urine, water, foods and other materials of sanitary importance. It is also used as an enrichment medium for the isolation of some species of shigella.
Selenite promotes good business practices and inspires honesty in business and professional relationships. Product summary and explanation selenite broth was originated by leifson,1 while observing good recovery of salmonella spp. Do not incubate the broth longer than 24 hours as inhibitory effect of selenite decreases after 6 12 hours of incubation 5. R454582 synonyms no information available recommended use laboratory chemicals. It is the most widely used enrichment medium to isolate salmonella typhi from fecal specimen of suspected patients andor from carriers during salmonella outbreaks. Pdf salmonella contamination of poultry food may cause increase of feed conversion and mortality rates, reduction of egg size, decrease in egg laying. Liefson 2 suggested that it is best to tube the medium to a depth of 2 inches 50mm or more. Mannitol selenite broth is a modification of leifsons recipe where mannitol is added to the broth to improve the recovery of salmonella as reported by hobbs and allison. Cm0395 risk phrases safety phrases ec classification this product is not classified according to the eu regulations.
The selective effect is not complete, for although the unwanted species are inhibited during the first 12 hours of incubation, they later. August 2012 made available in pdf format versions of chapter 5. Puritan selenite broth transport medium intended use puritan selenite broth transport medium is a selective enrichment medium used for the isolation of salmonella spp and shigella spp. Selenite f broth is the medium used for the selective enrichment of salmonella spp from both clinical and food samples. Selenite cystine broth 7283 intended use selenite cystine broth is used for the selective enrichment of salmonella species in a laboratory setting. Product summary and explanation the growth and isolation of salmonella in food and other samples can be suppressed from foodprocessing procedures including exposure to low and or high temperatures, drying, radiation. Get a printable copy pdf file of the complete article 897k, or click on a page image below. Our handy tool even allows you to automatically split individual pages of your document into separate files.
Selenite broth 7155 intended use selenite broth is used for the selective enrichment of salmonella spp. Selenite cystine broth base selective enrichment medium for salmonella directions. Summary and explanation salmonella is an important bacterial pathogen of foodborne illness, ranking just behind c. Rearrange individual pages or entire files in the desired order. Sugar and gelatin in medium may get decomposed on autoclaving, hence they are exposed to free steaming for 20 minutes for three successive days. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. Rv medium replaces selenite cystine sc broth for the analysis of all. What links here related changes upload file special pages permanent link page. Selenite cystine broth dehydrated culture media for. When you have all these things going for you, it will be easy to succeed in any project or endeavor. Remel selenite cystine broth thermo fisher scientific. Rv medium made from its individual ingredients and incubated at 42c was compared with a commercial preparation of sc broth, incubated at 35c, and tt broth. Sodium selenite has been utilized in studies of cell proliferation and cancer research. Selenite broth is not intended for use in the diagnosis of disease or other conditions in humans.
Selenite f broth and the related medium, selenite cystine broth, are recommended for use in the recovery of salmonella with subcultures being made after 1218 h incubation. The selective effect is not complete, for although the unwanted species are inhibited during the first 12 hours of incubation, they later increase rapidly. Remel and oxoid products are now part of the thermo scientific brand, combining powerful manual, semiautomated and fully automated test. Selenite cystine broth is not intended for use in the diagnosis of disease or other conditions in humans. The efficiency of selenite broth of different compositions in the. Selenite broth was originally developed by liefson, and has for long been used as a standard method for the selective recovery of salmonella with the inhibition of other enteric microorganisms such as e. Original article evaluation of selenite f broth as an. Following 1824 hours of incubation, the selenite broth is plated to he or xld using same technique as above. Copan selenite broth is an enrichment selective broth for clinical specimens. Selenite broth selenite f broth twin pack m052 intended use.
Tetrathionate broth is used for selective enrichment of salmonella. Relative effectiveness of selenite cystine broth, tetrathionate broth, and rappaportvassiliadis medium for the. Selenite cystine enrichment broth 107709 merck millipore. Direct culture on hektoen agar yielded a recovery rate of 39% of the salmonella isolated and 67% of the shigella isolated. Composition ingredients gms litre part a tryptone 5. Summary and explanation leifson found that selenite inhibited fecal streptococci and coliforms during the first 812 hours of incubation, thereby. Remove the need for pipetting, dispensing or autoclaving with convenient, prefilled and readytouse tubes of selenite broth. Mwe selenite broth product code description mwsel 2ml of selenite broth in vial with white screw cap 1. Selenite broth exist, including selenite cystine broth, from the original formula described as selenite f broth by leifson. Get a printable copy pdf file of the complete article 340k, or click on a page image below to browse page by page.
Peptone mixture is a source of nitrogen, vitamins and amino acids essential for growth. It can also be used for water, foods for the isolation of salmonella species. Selenite f broth was devised by leifson, who demonstrated that selenite was inhibitory for coliforms and certain other microbial species. Pdf rfactor transfer in selenite and tetrathionate broths. Selenite broth is inhibitory and recommended for selective isolation of salmonella species. Relative effectiveness of selenite cystine broth, tetrathionate broth. Split pdf pdf split into multiple files online free. Selenite broth is a selective medium which is inhibitory to organisms such as e. This process is known as tyndallisation after john tyndall or fractional sterilization or intermittent sterilization. Selenite broth is used as a selective enrichment for the cultivation of salmonella spp.
Help your pdf meet the size limit by breaking down large documents into smaller pieces to facilitate electronic distribution. Pi7155, rev 04, april 2008 selenite broth 7155 intended use selenite broth is used for the selective enrichment of salmonella spp. Pi7155, rev 02, 122303 selenite broth 7155 intended use selenite broth is used for the selective enrichment of salmonella spp. Cled agar is an abbreviation for cystine lactoseelectrolytedeficient agar. Selenite f broth principle, uses, preparation and result. Remel selenite broth w and wo cystine are liquid media recommended for use in. Other companies are charging thousands of dollars to set up accounts and give you access to their msds online database. Do not incubate the broth longer than 24 hours as inhibitory effect of selenite reduces after 612 hours of incubation 10. This quality assurance testing conforms with or exceeds clsi standards. All lot numbers of selenite broth w and wo cystine have been tested using the following quality control organisms and have been found to be acceptable. Puritan selenite broth transport medium intended use. Pi7283 rev 03, 012904 selenite cystine broth 7283 intended use selenite cystine broth is used for the selective enrichment of salmonella species. The broth is also recommended for the transport of strains of vibrio cholerae, because these organisms can survive 2 to 5 days in sodium selenite broth. It can be used for research of salmonella spp in stool samples or others biological materials.
Description selenite broth 2ml in 12x80mm screw cap tube in bulk pack size 50 pcs per box 6x50 per case. Selenite broth base safety data sheet date of issue 102008. Comparisons showed that mannitol selenite broth was superior to. Full text is available as a scanned copy of the original print version. Summary and explanation selenite broth was devised by leifson,1 who demonstrated that. It is a buffered lactose peptone broth to which sodium biselenite is added as the selective agent. Available formats pdf please select a format to send. Strontium chloride and strontium selenite enrichment broth media in the isolation of salmonella volume 67 issue 3 j. Selenite broth is an enrichment medium used for the selective isolation of salmonella and some species of shigella. Recommended as enrichment media for the isolation of salmonellae from faeces, urine or other pathological materials.
Media such as tcbs, dca and selenite broth are sterilized by steaming. Pdf direct culture into tetrathionate broth is better than iso 6579. Selenite broth was originated by leifson, while observing good recovery of salmonella spp. Sbg broth base selenite brilliant green broth base, for isolation of salmonella directions. Selenite broth selenite f broth twin pack himedia laboratories. Intended use mwe selenite broth is an enrichment medium for the isolation of salmonella species from faecal specimens. Pdf documents are often used because they help preserve the original content of the document, but this can make splitting the file up a little more difficult than other document formats. Selenite f broth is used as a selective enrichment for the cultivation of salmonella spp. Selenite f broth eb02e typical formula grams per litre peptone 5. Kelly, brenner and farmer, 2003, manual of clinical microbiology, 8th ed.
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